Chitrangada Das Mukhopadhyay
Email : Chitrangadadas@yahoo.com
Academic Details :
Previous Experience : Assistant Professor, Bengal Engineering and Science University, Shibpur,Howrah
Area of Work : In Vitro Detoxification of Aflatoxin B1 by Horse Radish peroxidase enzyme
The ability of horse radish peroxidase enzyme to degrade several xenobiotics was employed to detoxify aflatoxin B1, a carcinogen. In this connection various kinetic and thermodynamic parameters for the detoxification reaction were studied and the reaction was optimized. A statistical model was proposed to predict maximum possible degradation in a specified condition. The detoxified product was tested for its toxicity and mutagenicity on microbial systems and nutritive value of treated meal was tested on male wister rats. The effects of detoxification were also tested on seed and pollen germination, somatic embryogenesis and plant cell suspension culture. The experimental results showed that the detoxified product could safely be used by living organisms.